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7UC5

HLA-A*03:01 binding "ILRGSVAHK" at 1.95Å resolution

Data provenance

Structure downloaded from PDB Europe using the Coordinate Server. Aligned to residues 1-180 of 1HHK2 using the CEALIGN3 function of PyMol4. Chain assigment using a Levenshtein distance5 method using data from the PDBe REST API6. Organism data from PDBe REST API. Data for both of these operations from the Molecules endpoint. Structure visualised with 3DMol7.

Information sections

Complex type

Class i with peptide

1. Beta 2 microglobulin
['B', 'E']
2. Class I alpha
HLA-A*03:01
['A', 'D']
3. Peptide
ILRGSVAHK
['C', 'F']

Species

Locus / Allele group

HLA-A / HLA-A*03

Publication

CD8+ T cells cross-recognise conserved influenza A and B homologous epitopes

Nguyen, A.T., Lau, H.M.P., Sloane, H., Jayasinghe, D., Mifsud, N.A., Chatzileontiadou, D.S.M., Grant, E.J., Szeto, C., Gras, S.

Structure deposition and release

Deposited: 2022-03-16
Released: 2022-10-12
Revised: 2022-10-12

Data provenance

Publication data retrieved from PDBe REST API8 and PMCe REST API9

Other structures from this publication

Peptide details

Length: Nonamer (9 amino acids)

Sequence: ILRGSVAHK

Interactive view
Cutaway side view (static)
Surface top view (static - coloured by atom property)
Cutaway top view (static)

Data provenance

MHC:peptide complexes are visualised using PyMol. The peptide is superimposed on a consistent cutaway slice of the MHC binding cleft (displayed as a grey mesh) which best indicates the binding pockets for the P1/P5/PC positions (side view - pockets A, E, F) and for the P2/P3/PC-2 positions (top view - pockets B, C, D). In some cases peptides will use a different pocket for a specific peptide position (atypical anchoring). On some structures the peptide may appear to sterically clash with a pocket. This is an artefact of picking a standardised slice of the cleft and overlaying the peptide.

Peptide neighbours

P1 ILE

THR163
GLU63
TYR171
MET5
TRP167
TYR159
TYR59
TYR7
GLN62
 P2 LEU

TYR99
PHE9
ASN66
TYR7
GLU63
MET45
VAL67
TYR159
 P3 ARG

ARG114
GLN155
TYR99
TYR159
ASN66
GLU152
LEU156
 P4 GLY

ASN66
 P5 SER

GLN155
 P6 VAL

ASN66
GLN70
ALA69
THR73
 P7 ALA

TRP147
GLU152
ASP77
THR73
 P8 HIS

TRP147
ASP77
LYS146
VAL76
THR73
 P9 LYS

TRP147
LEU81
ILE142
ASP77
THR80
TYR84
ILE95
THR143
TYR123
ARG114
LYS146
ILE124
ILE97
ASP116

Colour key

Aromatic Hydrophobic Acidic Basic Neutral/polar

Data provenance

Neighbours are calculated by finding residues with atoms within 5Å of each other using BioPython Neighboursearch module. The list of neighbours is then sorted and filtered to inlcude only neighbours where between the peptide and the MHC Class I alpha chain.

Colours selected to match the YRB scheme. [https://www.frontiersin.org/articles/10.3389/fmolb.2015.00056/full]

Binding cleft pockets


Peptide sidechain binding pockets (static)
Peptide terminii and backbone binding residues (static)
A Pocket

TYR159
THR163
TRP167
TYR171
MET5
TYR59
GLU63
ASN66
TYR7
B Pocket

ALA24
VAL34
MET45
GLU63
ASN66
VAL67
TYR7
GLN70
PHE9
TYR99
C Pocket

GLN70
THR73
ASP74
PHE9
ILE97
D Pocket

ARG114
GLN155
LEU156
TYR159
LEU160
TYR99
E Pocket

ARG114
TRP147
GLU152
LEU156
ILE97
F Pocket

ASP116
TYR123
THR143
LYS146
TRP147
ASP77
THR80
LEU81
TYR84
ILE95

Colour key

Binds N-terminus Binds P1 backbone Binds P2 backbone Binds PC-1 backbone Binds C-terminus

Data provenance

N-/C-terminus and peptide backbone binding residues are assigned according to previously published information and pockets are assigned according to an adaptation of a previously published set of residues. All numbering is currently that of the 'canonical' structures of human and mouse MHC Class I molecules.

Chain sequences

1. Beta 2 microglobulin
Beta 2 microglobulin
        10        20        30        40        50        60
MIQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKD
        70        80        90
WSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM
2. Class I alpha
HLA-A*03:01
IPD-IMGT/HLA
[ipd-imgt:HLA34773]
        10        20        30        40        50        60
GSHSMRYFFTSVSRPGRGEPRFIAVGYVDDTQFVRFDSDAASQRMEPRAPWIEQEGPEYW
        70        80        90       100       110       120
DQETRNVKAQSQTDRVDLGTLRGYYNQSEAGSHTIQIMYGCDVGSDGRFLRGYRQDAYDG
       130       140       150       160       170       180
KDYIALNEDLRSWTAADMAAQITKRKWEAAHEAEQLRAYLDGTCVEWLRRYLENGKETLQ
       190       200       210       220       230       240
RTDPPKTHMTHHPISDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDGT
       250       260       270
FQKWAAVVVPSGEEQRYTCHVQHEGLPKPLTLRWELS
3. Peptide
ILRGSVAHK

Data provenance

Sequences are retrieved via the Uniprot method of the RSCB REST API. Sequences are then compared to those derived from the PDB file and matched against sequences retrieved from the IPD-IMGT/HLA database for human sequences, or the IPD-MHC database for other species. Mouse sequences are matched against FASTA files from Uniprot. Sequences for the mature extracellular protein (signal petide and cytoplasmic tail removed) are compared to identical length sequences from the datasources mentioned before using either exact matching or Levenshtein distance based matching.

Downloadable data

Data can be downloaded to your local machine from the links below.
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   e.g. load http://www.histo.fyi/structures/downloads/1hhk_1_peptide.cif
or in the case of JSON formatted files to retrieve it and use it as part of notebooks such as Jupyter or GoogleColab.
Please take note of the data license. Using data from this site assumes that you have read and will comply with the license.

Complete structures

Aligned structures [cif]
  1. 7UC5 assembly 1  
  2. 7UC5 assembly 2  

Components

MHC Class I alpha chain [cif]
  1. 7UC5 assembly 1  
  2. 7UC5 assembly 2  
MHC Class I antigen binding domain (alpha1/alpha2) [cif]
  1. 7UC5 assembly 1  
  2. 7UC5 assembly 2  
Peptide only [cif]
  1. 7UC5 assembly 1  
  2. 7UC5 assembly 2  

Derived data

Data for this page [json]
https://api.histo.fyi/v1/structures/7uc5

Data license

The data above is made available under a Creative Commons CC-BY 4.0 license. This means you can copy, remix, transform, build upon and redistribute the material, but you must give appropriate credit, provide a link to the license, and indicate if changes were made.
If you use any data downloaded from this site in a publication, please cite 'https://www.histo.fyi/'. A preprint is in preparation.

Footnotes


Creative Commons Licence
This work is licensed under a Creative Commons Attribution 4.0 International License.