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6UON

HLA-C*08:02 presenting "GADGVGKSAL" to Alpha/Beta T cell receptor at 3.50Å resolution

Data provenance

Structure downloaded from PDB Europe using the Coordinate Server. Aligned to residues 1-180 of 1HHK2 using the CEALIGN3 function of PyMol4. Chain assigment using a Levenshtein distance5 method using data from the PDBe REST API6. Organism data from PDBe REST API. Data for both of these operations from the Molecules endpoint. Structure visualised with 3DMol7.

Information sections

Complex type

Class i with peptide and alpha beta tcr

1. Beta 2 microglobulin
['B', 'E']
2. Class I alpha
HLA-C*08:02
['A', 'D']
3. Peptide
GADGVGKSAL
['C', 'F']
4. T cell receptor alpha
TRAV12
['G']
5. T cell receptor beta
TRBV10
['H']

Species

Locus / Allele group

HLA-C / HLA-C*08

Publication

High-affinity oligoclonal TCRs define effective adoptive T cell therapy targeting mutant KRAS-G12D.

Sim MJW, Lu J, Spencer M, Hopkins F, Tran E, Rosenberg SA, Long EO, Sun PD
Proc. Natl. Acad. Sci. U.S.A. (2020) [doi:10.1073/pnas.1921964117]  [pubmed:32461371] 

Structure deposition and release

Deposited: 2019-10-15
Released: 2020-05-27
Revised: 2020-06-17

Data provenance

Publication data retrieved from PDBe REST API8 and PMCe REST API9

Other structures from this publication

Peptide details

Length: Decamer (10 amino acids)

Sequence: GADGVGKSAL

Interactive view
Cutaway side view (static)
Surface top view (static - coloured by atom property)
Cutaway top view (static)

Data provenance

MHC:peptide complexes are visualised using PyMol. The peptide is superimposed on a consistent cutaway slice of the MHC binding cleft (displayed as a grey mesh) which best indicates the binding pockets for the P1/P5/PC positions (side view - pockets A, E, F) and for the P2/P3/PC-2 positions (top view - pockets B, C, D). In some cases peptides will use a different pocket for a specific peptide position (atypical anchoring). On some structures the peptide may appear to sterically clash with a pocket. This is an artefact of picking a standardised slice of the cleft and overlaying the peptide.

Peptide neighbours

P1 GLY

TYR7
TYR171
TYR159
TYR59
LYS66
TYR99
GLU63
TRP167
MET5
 P10 LEU

LYS146
TYR123
THR143
LEU81
LEU95
ASN80
TRP147
SER77
TYR84
 P2 ALA

TYR99
TYR159
GLU63
LYS66
TYR9
TYR67
TYR7
 P3 ASP

GLN70
TYR99
TYR159
ASN114
TYR9
ARG97
ARG156
 P4 GLY

ARG69
 P5 VAL

TYR159
GLN155
ARG156
 P6 GLY

GLN155
ARG156
 P7 LYS

THR73
GLN155
GLU152
 P8 SER

ARG156
THR73
ASP74
PHE116
TRP147
SER77
GLU152
GLN70
 P9 ALA

THR73
TRP147
SER77

Colour key

Aromatic Hydrophobic Acidic Basic Neutral/polar

Data provenance

Neighbours are calculated by finding residues with atoms within 5Å of each other using BioPython Neighboursearch module. The list of neighbours is then sorted and filtered to inlcude only neighbours where between the peptide and the MHC Class I alpha chain.

Colours selected to match the YRB scheme. [https://www.frontiersin.org/articles/10.3389/fmolb.2015.00056/full]

Binding cleft pockets


Peptide sidechain binding pockets (static)
Peptide terminii and backbone binding residues (static)
A Pocket

TYR159
THR163
TRP167
TYR171
MET5
TYR59
GLU63
LYS66
TYR7
B Pocket

ALA24
VAL34
GLY45
GLU63
LYS66
TYR67
TYR7
GLN70
TYR9
TYR99
C Pocket

GLN70
THR73
ASP74
TYR9
ARG97
D Pocket

ASN114
GLN155
ARG156
TYR159
LEU160
TYR99
E Pocket

ASN114
TRP147
GLU152
ARG156
ARG97
F Pocket

PHE116
TYR123
THR143
LYS146
TRP147
SER77
ASN80
LEU81
TYR84
LEU95

Colour key

Binds N-terminus Binds P1 backbone Binds P2 backbone Binds PC-1 backbone Binds C-terminus

Data provenance

N-/C-terminus and peptide backbone binding residues are assigned according to previously published information and pockets are assigned according to an adaptation of a previously published set of residues. All numbering is currently that of the 'canonical' structures of human and mouse MHC Class I molecules.

Chain sequences

1. Beta 2 microglobulin
Beta 2 microglobulin
        10        20        30        40        50        60
IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDW
        70        80        90
SFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM
2. Class I alpha
HLA-C*08:02
IPD-IMGT/HLA
[ipd-imgt:HLA35375]
        10        20        30        40        50        60
CSHSMRYFYTAVSRPGRGEPRFIAVGYVDDTQFVQFDSDAASPRGEPRAPWVEQEGPEYW
        70        80        90       100       110       120
DRETQKYKRQAQTDRVSLRNLRGYYNQSEAGSHTLQRMYGCDLGPDGRLLRGYNQFAYDG
       130       140       150       160       170       180
KDYIALNEDLRSWTAADKAAQITQRKWEAAREAEQRRAYLEGTCVEWLRRYLENGKKTLQ
       190       200       210       220       230       240
RAEHPKTHVTHHPVSDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDGT
       250       260       270
FQKWAAVVVPSGEEQRYTCHVQHEGLPEPLTLRW
3. Peptide
GADGVGKSAL
4. T cell receptor alpha
T cell receptor alpha
TRAV12
        10        20        30        40        50        60
MKEVEQNSGPLSVPEGAIASLNCTYSDRGSQSFFWYRQYSGKSPELIMFIYSNGDKEDGR
        70        80        90       100       110       120
FTAQLNKASQYVSLLIRDSQPSDSATYLCAAAMDSSYKLIFGSGTRLLVRPDIQNPDPAV
       130       140       150       160       170       180
YQLRDSKSSDKSVCLFTDFDSQTNVSQSKDSDVYITDKCVLDMRSMDFKSNSAVAWSNKS
       190       200
DFACANAFNNSIIPEDTFFPSP
5. T cell receptor beta
T cell receptor beta
TRBV10
        10        20        30        40        50        60
MAGITQSPRYKITETGRQVTLMCHQTWSHSYMFWYRQDLGHGLRLIYYSAAADITDKGEV
        70        80        90       100       110       120
PDGYVVSRSKTENFPLTLESATRSQTSVYFCASSDPGTEAFFGQGTRLTVVEDLRNVFPP
       130       140       150       160       170       180
EVAVFEPSEAEISHTQKATLVCLATGFYPDHVELSWWVNGKEVHSGVCTDPQPLKEQPAL
       190       200       210       220       230       240
NDSRYALSSRLRVSATFWQNPRNHFRCQVQFYGLSENDEWTQDRAKPVTQIVSAEAWGRA

D

Data provenance

Sequences are retrieved via the Uniprot method of the RSCB REST API. Sequences are then compared to those derived from the PDB file and matched against sequences retrieved from the IPD-IMGT/HLA database for human sequences, or the IPD-MHC database for other species. Mouse sequences are matched against FASTA files from Uniprot. Sequences for the mature extracellular protein (signal petide and cytoplasmic tail removed) are compared to identical length sequences from the datasources mentioned before using either exact matching or Levenshtein distance based matching.

Downloadable data

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Complete structures

Aligned structures [cif]
  1. 6UON assembly 1  

Components

MHC Class I alpha chain [cif]
  1. 6UON assembly 1  
MHC Class I antigen binding domain (alpha1/alpha2) [cif]
  1. 6UON assembly 1  
Peptide only [cif]
  1. 6UON assembly 1  

Derived data

Data for this page [json]
https://api.histo.fyi/v1/structures/6uon

Data license

The data above is made available under a Creative Commons CC-BY 4.0 license. This means you can copy, remix, transform, build upon and redistribute the material, but you must give appropriate credit, provide a link to the license, and indicate if changes were made.
If you use any data downloaded from this site in a publication, please cite 'https://www.histo.fyi/'. A preprint is in preparation.

Footnotes


Creative Commons Licence
This work is licensed under a Creative Commons Attribution 4.0 International License.